Clinical Significance
Vitamin D, 25-Hydroxy, Total, Immunoassay - Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia
Preferred Specimen(s)
0.8 mL serum
Minimum Volume
0.5 mL
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria
Moderate hemolysis • Grossly lipemic • Plasma
Patient Preparation
Fasting preferred, but not required
Methodology
Immunoassay (IA)
Reference Range(s)
30-100 ng/mL
Alternative Name(s)
25 OH Vitamin D,Vitamin D, 25-OH,25-Hydroxy Vitamin D,Vitamin D, 25-Hydroxy