ESTRADIOL

"Clinical Significance
Estradiol - Measuring the circulating levels of estradiol is important for assessing the ovarian function and monitoring follicular development for assisted reproduction protocols. Estradiol plays an essential role throughout the human menstrual cycle. Elevated estradiol levels in females may also result from primary or secondary ovarian hyperfunction. Very high estradiol levels are found during the induction of ovulation for assisted reproduction therapy or in pregnancy. Decreased estradiol levels in females may result from either lack of ovarian synthesis (primary ovarian hypofunction and menopause) or a lesion in the hypothalamus-pituitary axis (secondary ovarian hypofunction). Elevated estradiol levels in males may be due to increased aromatization of androgens, resulting in gynecomastia.

Preferred Specimen(s)
1 mL serum

Minimum Volume
0.5 mL

Transport Container
Serum Separator Tube (SST®)

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 6 months
*Do not add estradiol testing to a barrier gel specimen older than 72 hours

Reject Criteria
Serum stored on barrier gel for more than 72 hours

Setup Schedule, Collection Instructions"

Methodology
Immunoassay (IA)

Reference Range(s)
Female     
  Follicular Phase    19-144 pg/mL
  Mid-Cycle    64-357 pg/mL
  Luteal Phase    56-214 pg/mL
  Postmenopausal    ≤31 pg/mL
Male    ≤39 pg/mL

Alternative Name(s)
Estradiol, 17 Beta,IVF Estradiol,17-Beta-Estradiol,E2 IVF,Estradiol (E2-6 III)